![]() ![]() Again credit for the original solution goes to the answer for Matplotlib overlapping annotations. With a lot of fiddling, I figured it out. Here is the example image: import matplotlib.pyplot as pltĪdjust_text(texts, only_move=, force_points=0.15,Īrrowprops=dict(arrowstyle="->", color='r', lw=0.5)) I just wanted to post here another solution, a small library I wrote to implement this kind of things: Īn example of the process can be seen here: Images (if this works) can be found here (this code): P1 = plt.plot(eucs,covers,color="black", alpha=0.5) Plt.ylabel("Percentage Timewindows Attended") Plt.xlabel("Proportional Euclidean Distance") I'm having trouble converting the "axis" methods over to what I want to do, and I don't understand how the text lines up. ![]() The method suggested in the accepted answer to Matplotlib overlapping annotations looks extremely promising, however is for bar graphs. I'm also using the test data that the creators of TOBIAS provide when running this command, so this error is likely not resulting from an error with the files I am providing as inputs to the command.I'm trying to stop annotation text overlapping in my graphs. I tried uninstalling and reinstalling matplotlib using pip ( pip uninstall matplotlib and pip install matplotlib, respectively), but I still get the same error. I tried upgrading matplotlib using pip install -upgrade matplotlib but I already have the latest version, 3.7.1. ![]() Text._init_(self, x, y, text, **kwargs)įile "/home/usrname/.local/lib/python3.10/site-packages/matplotlib/_api/deprecation.py", line 454, in wrapperįile "/home/usrname/.local/lib/python3.10/site-packages/matplotlib/text.py", line 183, in _init_įile "/home/usrname/.local/lib/python3.10/site-packages/matplotlib/text.py", line 231, in updateįile "/home/usrname/.local/lib/python3.10/site-packages/matplotlib/artist.py", line 1213, in updateįile "/home/usrname/.local/lib/python3.10/site-packages/matplotlib/artist.py", line 1197, in _update_propsĪttributeError: 'Annotation' object has no property 'text_from_points' 08:54:45 (488) - Bcell / Tcell (static plot)įile "/home/usrname/.local/bin/TOBIAS", line 8, in įile "/home/usrname/.local/lib/python3.10/site-packages/tobias/TOBIAS.py", line 154, in mainįile "/home/usrname/.local/lib/python3.10/site-packages/tobias/tools/bindetect.py", line 819, in run_bindetectįig = plot_bindetect(motif_list, clustering,, args)įile "/home/usrname/.local/lib/python3.10/site-packages/tobias/tools/bindetect_functions.py", line 769, in plot_bindetectĪdjust_text(txts, ax=ax1, add_objects=, text_from_points=True, arrowprops=dict(arrowstyle='-', color='black', lw=0.5)) #, expand_text=(0.1,1.2), expand_objects=(0.1,0.1))įile "/home/usrname/.local/lib/python3.10/site-packages/adjustText/_init_.py", line 730, in adjust_textįile "/home/usrname/.local/lib/python3.10/site-packages/matplotlib/axes/_axes.py", line 699, in annotateĪ = mtext.Annotation(text, xy, xytext=xytext, xycoords=xycoords,įile "/home/usrname/.local/lib/python3.10/site-packages/matplotlib/text.py", line 1885, in _init_ However, I get the following error in my output: 08:54:45 (488) Writing all_bindetect files TOBIAS BINDetect -motifs test_data/motifs.jaspar -signals test_data/Bcell_footprints.bw test_data/Tcell_footprints.bw -genome test_data/genome.fa -peaks test_data/merged_peaks_annotated.bed -peak_header test_data/merged_peaks_annotated_header.txt -outdir BINDetect_test -cond_names Bcell Tcell -cores 4 I tried to run the last step of the pipeline, BINDetect, using the following command: ![]() I am attempting to run TOBIAS, a collection of command-line bioinformatics tools, to analyze my sequencing data from ATAC-seq. ![]()
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![]() On October 27, 2020, ICE released a revised version of the PRR, which updated the definition of and procedures surrounding severe psychiatric illness in ICE’s identification of aliens at higher risk of severe illness from COVID-19 adjusted ICE procedures in notifying the detainee and his or her legal counsel within 12 hours that the detainee falls within the populations identified as potentially being at higher risk of severe illness from COVID-19 and/or subclasses certified in Fraihat v.
![]() ![]() Because their WSDL isn’t 100% compatible with. Luckily, provides a geo-coding service free of charge in the form of a web service. ) and turning it into a pair of latitude/longitude values. Geocoding is the process of taking a address ( 123 Main Street, Denver, CO In other words, you create appointments and anyone can see a map or satellite images of where you are at any given time! That server also hosts a web site to allow anyone to view all scheduled appointmentts and see a map of where the appointment is physically located. Then I’m using a custom built web service to save scheduled appointments and lat/long values to a server. I’m relying on the web service to turn an address into the needed latitude and longitude values. Part of our Smart Client definition the ability to connect to distributed data sources. The problem is that Google’s mapping API requires latitude and longitude values to generate a map, and they don’t have a geo-coding service to turn an address into latitude and longitude. Then, that appointment could be mapped using Google’s mapping service. The idea for the application is to allow a user to enter appointments (similar to Outlook), but incorporate an address or intersection for the location of the appointment. I thought it would be interesting to combine a Smart Client application with Google’s mapping. Google recently opened their mapping API to developers. ![]() You can read here for a comprehensive explanation.īefore we get going, feel free to check out to get an idea of the end result for this application. This is the definition I’ll be following throughout this article to, and the definition on which this application is built. ![]() “Smart clients are easily deployed and managed client applications that provide an adaptive, responsive and rich interactive experience by leveraging local resources and intelligently connecting to distributed data sources.” A solid definition is the foundation for a Smart Cliet’s architecture and design choices. The first order of business is understanding the nature of a Smart Client application. I'm using this competition as a way to solve all three problems. Third, my cell phone is dieing and I could really use one that works.Įnter the CodeProject Smart Client Competition Second, I need to start learning all the new features of the. First, the “Smart Client” buzz is beginning to dominate the technology charts and I want to have a better understanding of this new paradigm. I have three problems I’ve been trying to solve for a while. ![]() ![]() The construction industry is much different. Most industries operate on annual reporting. Standard accounting systems can’t handle construction financial processes Damen Edwards from Construction Management Podcast " What makes construction financial management different from the financial management of companies outside the construction industry? Knowing where the money is going and how to allocate that money will separate a good construction manager from a great construction manager. That's why putting all your financial management on manual work is doomed to fail from the beginning. The human eye cannot catch an error in loads of financial data. Still, many construction companies manage their finances only in spreadsheets. Higher risk of manual errorsĭid you know there's a 40% chance of committing an error while manually entering data into spreadsheets? The risk reaches 100% if you deal with complex files. You can't see how decisions impact your company which means you have zero control over what's happening with your business. With many detailed worksheets, you lack the crucial aspect: an overview of the company's financial health. They don't see that operating only on workarounds is the nail in the coffin rather than the key to success. Especially when the alternative is a pricey system. Workarounds for many construction managers seem to be a faster and cheaper way of managing finances. If you spend your time creating contemporary worksheets, you have no time left for actions that matter- like winning more businesses. ![]() In the 21st century, construction companies must be fast and efficient to stay on the market. After all, you must take the financial data from a primary source, create a workaround and manage the information. But, basing your whole construction project's financial management on them is not a good strategy: Waste of your time and energyīuilding workaround spreadsheets every time costs you time and energy. Workarounds might be a good solution for managing risks or unpredicted changes. The importance of doing construction financial management right, with no workarounds Yet, to be able to analyse your situation, you need to gather, organise and learn how to manage financial data. That's why in proper financial management, every process matters, from small purchases to structural changes on a business level. ![]() This is how managing your construction finances looks inside ArchdeskĬonstruction financial management is allocating and accounting for financial resources to cut project costs, maximise profits and assure long-term company financial health.Įvery decision impacts your financial position. What is meant by construction financial management? In this guide, you'll find answers to the most common questions with tips and best practices that will help you ensure your company's financial safety. ![]() Do you feel your techniques and tools for construction financial management aren't enough?ĭo you sometimes go over budget due to poorly estimated project costs?ĭo you face problems with managing your company's cash flow? ![]() ![]() ![]() This amount of cargo space is the highest in the segment. The cargo capacity of the 2020 Sprinter is its strongest utility and it provides a solid 319 ft³ of cargo volume. The cabin also does a good job of giving a serene atmosphere inside eliminating mostly all exterior noise save the usual coarse engine noise. Some additional features worth noting are the 180 degrees opening back doors and the efficient climate control that makes for a really comfortable travel experience on hot summer days. ![]()
![]() Image courtesy of Where to Find an Incubator This add to the hygienic environment into which the chicks are born resulting in healthier chickens from day one. A recent development in egg incubators is the addition of a permanent antibacterial additive that gets added into the plastic that provides maximum protection during the lifetime of the product. ![]() However, these incubators tend to be expensive, and may not be worth the investment unless you plan on incubating chicken eggs on a regular basis. You just place the eggs inside and wait 21 days, the incubation period for chicken eggs, till the first chick pips. Which features are most important to you will depend on how much input you want to have in the process of if you want a “set and forget” egg incubator.įully automated egg incubators will take all the stress out of hatching chicken eggs. Egg incubators with turners will save you more time than any other feature. Essentially, if you are only going to hatch one batch from your egg incubator a cheap incubator might do quite nicely but if you are planning on hatching multiple times then consider a more advanced option.Įven a simple egg incubator can usually be purchased with add-ons, like automated egg turners or fans. There is a specific temperature and humidity for hatching chicken eggs and you will have to maintain these yourself, as well as turning the eggs several times a day. But these egg incubators, typically made of Styrofoam, are all manual. There are a few things to consider when choosing an incubator:Ĭheap incubators for hatching eggs are appealing to many people on their first hatch. For tips on successful hatching using an egg incubator, check out our Rules for Hatching Chicken Eggs. This article will help you purchase or build the best chicken egg incubator. Incubating chicken eggs is a rewarding experience, but it does require some special equipment and a little know-how. Perhaps you don’t have a broody hen or a safe space for her to raise her chicks, maybe you want to hatch more eggs than a hen can set on or perhaps you want to involve your children in the experience in a more hands-on way. There are many reasons that you might choose to hatch eggs artificially using an egg incubator. ![]() ![]() Marine-grade, UV-resistant, nylon stitching with French hem styling.Classic Journal style elastic strap closure!.Perfectly fits the iPhone 12 Pro with 6.1-inch screen.Wood Tray is removable with our 3M Clean Release Adhesive!.Full Grain Leather Wallet cover with room for 5-7 cards and cash.12% more compact than previous wallet versions.Hewn from a single piece of FSC certified Cherry hardwood.If you can wait that long, check it out.All NEW LeatherSafe® Luxury Book iPhone 12 Pro Wallet Case The only reason, it is low on the list is that it’s on back-order and has a 14 day lead time for delivery. If you want both style and substance, buy this one. ![]() And right out of the box, I think this is the best-looking leather case on this list. The body of the case is wrapped inside supple Horween leather which feels premium to touch and develops a natural patina over time. The case uses a high-grade polycarbonate body, an internal shock absorption bumper, and a raised TPE bumper along the perimeter of the screen, to provide the best drop protection that you can hope for. The Nomad Rugged Case for iPhone 12 Pro is without a doubt one of the best leather cases that you can buy. I love Nomad’s product and their leather cases are my jam. Nomad Rugged Case for iPhone 12 ProĪnother favorite leather case maker of mine is Nomad. If the price is not a factor for you, you should buy this one.īuy from Amazon: $75 7. Not to mention, it has a soft microfiber lining on the inside that keeps the scratches and scuffs at bay. The case is also extra protective and provides good drop protection. The card enclosure is covered using a magnetic flap that reduces the chances of your card falling out of the case. The case also has a built-in wallet for your cards (fit up to 3 cards). ![]() ESR became the first company to release a MagSafe wireless car charger for the iPhone 12 Pro, and now the company has released some really cool cases and screen protectors. If you are looking for a budget leather case that uses actual leather and not synthetic leather, check out the ESR premium leather case. The only drawback of this sleeve is that it’s quite costly, coming at $129.īuy from Apple: $129 5. The Apple Leather sleeve is also compatible with MagSafe technology so, you don’t have to take out the phone for charging. It also comes with a matching strap that you can use for extra grip. The sleeve has a built-in card holder inside that you can use to hold cards and cash. Just like the leather case, the leather sleeve is made of tanned and finished European leather which feels soft to touch and develops a natural patina over time. It covers the iPhone from all sides, including the camera bump. If you want all-around protection for your iPhone 12 Pro, check out this leather sleeve from Apple. It’s available in two colors at launch Black and Tan.Ĥ. ![]() The built-in wallet can handle up to three credit cards and cash. The case is also pretty slim and doesn’t add much bulk to the device. Mine has handled drops like a champ, and I couldn’t have been happier. The case has not shown any sign of wear (apart from the scratches that it incurred due to falls), and the natural patina has developed beautifully.įrom my experience, I can tell you that Mujjo cases can easily protect your brand new iPhone 12 and iPhone 12 pro. I have been using the Mujjo wallet leather case on my iPhone X for the past three years, and I love it. When it comes to premium leather cases, Mujjo cases are my favorite. Mujjo Full Leather Wallet Case for iPhone 12 Pro OtterBox Strada Series Case for iPhone 12 Proġ. Salawat Compatible with iPhone 12 Pro Caseīellroy iPhone 12 Pro Leather Case with Card Holder ![]() ![]()
![]() ![]() ![]() Head inside to vote and let us know who Britney Spears should collaborate with next. So we put together a poll and that's where you come in! She has even previously expressed interest in working with Justin Timberlake again, and we can totally picture a second Madonna duet! That's barely scratching the surface of viable options.įrankly, there are too many for us to choose from. Should she hop in the studio with Rihanna? What about Lady Gaga or Ariana Grande or Dua Lipa? These singles got us thinking, and we started putting together a list of artists that we would love to see Britney duet with in the future. Now, she is reunited with "Scream & Shout" collaborator Will.i.am to drop a new song called "Mind Your Business." However, she returned to the charts (and made history) alongside Elton John with their hit "Hold Me Closer" in 2022. The 41-year-old Princes of Pop hasn't released an album since Glory in 2016. Other fan accounts soon followed and Mason's fake Styles TikTok account began to gain attention from pop culture news sites and Styles fan communities.Britney Spears appears to be making a musical comeback, and we want to know who you think she should work with next. All this was going to be wasted."īut then on February 25, HSDaily - a Twitter fanpage with 500,000 followers - tweeted out the video. "I didn't think anyone was going to believe me. "At this point, all hope was lost," Mason said in a YouTube video he made explaining the prank. Harry Styles heads out of a local eatery after lunching with some friends in West Hollywood, Calif., on Wednesday afternoon (March 12). He then resorted to sending the plaque TikToks to various Harry Styles fanpages in the hopes that they would share it to their audiences, and he posted the TikTok to a Styles Twitter fanpage he had previously created. He made sure the picture captured his phone open in his hand with TikTok open, to make viewers think Styles was active on the platform.Īfter none of this content gained traction, Mason rented a studio and made a couple of professional-looking TikToks with the plaque. He bought clothing to match Styles' attire and enlisted a friend to snap photos of him walking with a bag holding a Spotify box. The page and commotion surrounding it became such a phenomenon that Newsweek, Highsnobiety, PopBuzz, and Rolling Stone wrote articles referencing the account. These speculative videos amassed hundreds of thousands of views on TikTok, and the hashtag "#suemonella" was viewed over 4.5 million times. Others devised intricate conspiracy theories about how the account's name "suemonella" could translate into Spanish as "dream of her," which they said could be a clue about an upcoming release. Fans matched up the clothing worn in the TikToks with Styles' past outfits and pointed out that Styles was allegedly recently captured in purported paparazzi photos carrying a bag with a big Spotify box. Suddenly, Styles' immense and dedicated fan base began to fixate on the account, with many convinced that the singer had set up a secret page to promote his next album. The account had barely any views, until it posted a video of a plaque that appeared to be from Spotify congratulating the singer Harry Style on reaching a billion streams on his song "Watermelon Sugar." It posted only a few videos: a hand reaching into a basket of fruit, legs walking up a pink staircase, and a montage of landscape shots with feet running. In February, a mysterious TikTok account spawned with no description, image, or information about its existence. Account icon An icon in the shape of a person's head and shoulders. ![]() ![]() ![]() The difference between the two successive terms is In the above example, we can see that a 1= 3 and a 2 = 5. The difference between the two successive terms is 2 therefore it is called the difference 'd'. The common form of an arithmetic sequence can be formulated as a n = a 1 + f × (n-1)įor Example, the sequence is 3, 5, 8, 11, 13, 15, 17……. This concentration increases a great deal during a PCR fortunately PCR seems quite robust for a variety of oligo melting temperatures.By using this Arithmetic Sequence Calculator, you can easily calculate the terms of an arithmetic sequence between two indices of this sequence in a few clicks. The value of this parameter is less than the actual concentration of oligos in the reaction because it is the concentration of annealing oligos, which in turn depends on the amount of template (including PCR product) in a given cycle. This parameter corresponds to 'c' in Rychlik, Spencer and Rhoads' equation (ii) (Nucleic Acids Research, vol 18, num 21) where a suitable value (for a lower initial concentration of template) is "empirically determined". ![]() The default (50nM) works well with the standard protocol used at the Whitehead/MIT Center for Genome Research-0.5 microliters of 20 micromolar concentration for each primer oligo in a 20 microliter reaction with 10 nanograms template, 0.025 units/microliter Taq polymerase in 0.1 mM each dNTP, 1.5mM MgCl2, 50mM KCl, 10mM Tris-HCL (pH 9.3) using 35 cycles with an annealing temperature of 56 degrees Celsius. ![]() Primer3 uses this argument to calculate oligo melting temperatures. Note that this is not the concentration of oligos in the reaction mix but of those annealing to template. The nanomolar concentration of annealing oligos in the PCR. You may need to choose more sensitive blast parameters (under advance parameters) if you want to detect targets with a higher number of mismatches than default. You can also lower the E value (see advanced parameters) in such case to speed up the search as the high default E value is not necessary for detecting targets with few mismatches to primers.Īdditionally this program has limit detecting targets that are too different from the will detect targets that have up to 35% mismatches to the primer sequences (i.e., a total of 7 mismatches for a 20-mer). For examaple, if you are only interested in targets that perfectly match the primers, you can set the value to 1. If the total number of mismatches between target and at least one primer (for a given primer pair) is equal to or more than the specified number (regardless of the mismatch locations), then any such targets will be ignored for primer specificity check. This is another parameter that can be used to adjust primer specificity stringecy. Try to lower the mismatch value in such case. However, specifying a larger mismatch value may make it more difficult to find such specific primers. The larger the mismatches (especially those toward 3' end) are between primers and the unintended targets, the more specific the primer pair is to your template (i.e., it will be more difficult to anneal to unintended targets). This requires at least one primer (for a given primer pair) to have the specified number of mismatches to unintended targets. You can use your own sequences (accession number, gi, or FASTA sequence) as a search database. This database is recommended if you are not considering variations represented by alternate loci. Mitochondrion and plastid genomes are also included where applicable.Īlthough sequences in this database are completely covered by the Refseq representative genomes database, it does not contain the alternate loci and thus avoids sequence redundancy introduced by including alternate loci. These are Refseq representative genomes from primary chromosome assemblies (i.e., no alternate loci) for many eukaryotic organisms. Genomes for selected eukaryotic organisms (primary assemblies only): This contains all RNA entries from NCBI's Reference Sequence collection Mitochondrion genomes are included where applicable. For other species, genomes from diverse isolates of the same species may be included. ![]() For the eukaryotes, only one genome is included per species (However, alternate loci of eukaryotic genomes are included where applicable). This database contains minimum redundancy in genome representation. These genomes are among the best quality genomes available at NCBI. This database contains NCBI RefSeq Reference and Representative genomes across broad taxonomy groups including eukaryotes, bacteria, archaea, viruses and viroids. This contains mRNA only from NCBI's Reference Sequence collection ![]() |
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